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The excitation of PerCP/Cy5.5 by 488 nm laser light induces a light emission maximum of 694 nm.$n$n$n

The excitation of APC/Cy7 by 647 nm laser light induces a light emission maximum of 785 nm.$n$n

The excitation of PE/Cy7 by 488 nm laser light induces a light emission maximum of 785 nm.$n$n

The excitation of PE/Cy3 by 488 nm laser light induces a light emission maximum of 578 nm.$n

DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. DTT is oftentimes used along with sodium dodecylsulfate